• VIRUPAKSHA BHAVANA Microbiology and Molecular Biology Lab, BioEdge Solutions, Karnataka, India.
  • BAPPALIGE SHAKUNTHALA Department of Botany, Sri Dharmasthala Manjunatheshwara College (Autonomous), Karnataka, India.
  • SHIRUR DAKAPPA SHRUTHI Microbiology and Molecular Biology Lab, BioEdge Solutions, Karnataka, India.


DNA barcoding, indoor plants, symbiotic bacteria, isolation, identification, molecular characterization


The health benefits of indoor plants are widely explored now a days and methods of psychological assessment are explored. In this regard we have collected few indoor plants and authenticated them to further investigate for its symbiotic relationship with bacteria. As traditional taxonomic studies possess intrinsic limitations with plant species identification, combinations of DNA barcodes have been considered a powerful tool to discover undetected genetic variation within species across large geographic areas, providing more precise estimates of biodiversity. Therefore, many genes have been targeted for DNA barcodes as standardizing regions is vital for identifying them efficiently and discriminate the plant species. In this study, we targeted different genes such as ITS2, matK, RbCl2 and PetB for sequencing. The plants were identified as Draceana senderiana,Pandanus sanderi and Hylotelephium ewersii. Once the species are authenticated they were further used to identify its bacterial relationship. The symbiotic bacteria present in roots were identified as Acinetobacter calcoaceticus and Pseudomonas stutzeri. This information will further help in exploring the properties of bacteria which are involved in enhancing the overall growth of plants.


Adachi, M., Rohde, C.L.E., Kendle, A.D. (2000): Effects of floral and foliage displays on human emotions. – HortTechnology 10(1): 59-63.

Barrett, L.G., Broadhurst, L.M., Thrall, P.H. (2012): Geographic adaptation in plant–soil mutualisms: tests using Acacia spp. and rhizobial bacteria. – Functional Ecology 26(2): 457-468.

CBOL Group, P.W., Hollingsworth, P.M., Forrest, L.L., Spouge, J.L., Hajibabaei, M., Ratnasingham, S., van der Bank, M., Chase, M.W., Cowan, R.S., Erickson, D.L., Fazekas, A.J. (2009): A DNA barcode for land plants. – Proceedings of the National Academy of Sciences 106(31): 12794-12797.

Chang, C.Y., Chen, P.K. (2005): Human response to window views and indoor plants in the workplace. – HortScience 40(5): 1354-1359.

Chase, M.W., Cowan, R.S., Hollingsworth, P.M., Van Den Berg, C., Madriñán, S., Petersen, G., Seberg, O., Jørgsensen, T., Cameron, K.M., Carine, M., Pedersen, N. (2007): A proposal for a standardised protocol to barcode all land plants. – Taxon 56(2): 295-299.

Dayrat, B. (2005): Towards integrative taxonomy. – Biological Journal Of The Linnean Society 85(3): 407-417.

Doyle, J.J., Doyle, J.L. (1987): A rapid DNA isolation procedure for small quantities of fresh leaf tissue. – Phytochemical Bulletin 19(1): 11-15.

Fazekas, A.J., Kuzmina, M.L., Newmaster, S.G., Hollingsworth, P.M. (2012): DNA barcoding methods for land plants. – In DNA barcodes, Humana Press, Totowa, NJ. 29p.

Frugier, F., Kosuta, S., Murray, J.D., Crespi, M., Szczyglowski, K. (2008): Cytokinin: secret agent of symbiosis. – Trends in Plant Science 13(3): 115-120.

Giudicelli, G.C., Mäder, G., Brandão de Freitas, L. (2015): Efficiency of ITS sequences for DNA barcoding in Passiflora (Passifloraceae). – International Journal of Molecular Sciences 16(4): 7289-7303.

Harman, G.E., Howell, C.R., Viterbo, A., Chet, I., Lorito, M. (2004): Trichoderma species-opportunistic, avirulent plant symbionts. – Nature Reviews Microbiology 2(1): 43-56.

Hebert, P.D., Cywinska, A., Ball, S.L., Dewaard, J.R. (2003): Biological identifications through DNA barcodes. – Proceedings of the Royal Society of London. Series B: Biological Sciences 270(1512): 313-321.

Lee, M.S., Lee, J., Park, B.J., Miyazaki, Y. (2015): Interaction with indoor plants may reduce psychological and physiological stress by suppressing autonomic nervous system activity in young adults: a randomized crossover study. – Journal of Physiological Anthropology 34(1): 1-6.

Lee, J., Tsunetsugu, Y., Takayama, N., Park, B.J., Li, Q., Song, C., Komatsu, M., Ikei, H., Tyrväinen, L., Kagawa, T., Miyazaki, Y. (2014): Influence of forest therapy on cardiovascular relaxation in young adults. – Evidence-Based Complementary and Alternative Medicine 2014: 7p.

Lee, J., Park, B.J., Tsunetsugu, Y., Ohira, T., Kagawa, T., Miyazaki, Y. (2011): Effect of forest bathing on physiological and psychological responses in young Japanese male subjects. – Public Health 125(2): 93-100.

Lohr, V.I., Pearson-Mims, C.H. (2005): Children's active and passive interactions with plants influence their attitudes and actions toward trees and gardening as adults. – HortTechnology 15(3): 472-476.

Meyer, C.P., Paulay, G. (2005): DNA barcoding: error rates based on comprehensive sampling. – PLoS Boil. 3(12): e422.

Orwell, R.L., Wood, R.A., Burchett, M.D., Tarran, J., Torpy, F. (2006): The potted-plant microcosm substantially reduces indoor air VOC pollution: II. Laboratory study. – Water, Air, and Soil Pollution 177(1): 59-80.

Park, B.J., Tsunetsugu, Y., Ishii, H., Furuhashi, S., Hirano, H., Kagawa, T., Miyazaki, Y. (2008): Physiological effects of Shinrin-yoku (taking in the atmosphere of the forest) in a mixed forest in Shinano Town, Japan. – Scandinavian Journal of Forest Research 23(3): 278-283.

Steinke, D., Zemlak, T.S., Boutillier, J.A., Hebert, P.D. (2009): DNA barcoding of Pacific Canada’s fishes. – Marine Biology 156(12): 2641-2647.

Tsunetsugu, Y., Park, B.J., Ishii, H., Hirano, H., Kagawa, T., Miyazaki, Y. (2007): Physiological effects of Shinrin-yoku (taking in the atmosphere of the forest) in an old-growth broadleaf forest in Yamagata Prefecture, Japan. – Journal of Physiological Anthropology 26(2): 135-142.

Vere, N.D., Rich, T., Trinder, S., Long, L. (2014): DNA barcoding for plant. – Methods in Molecular Biology 1245: 101-118.




How to Cite

BHAVANA, V., SHAKUNTHALA, B., & SHRUTHI, S. D. (2021). DNA BARCODING OF FEW INDOOR PLANTS AND MOLECULAR CHARACTERIZATION OF ITS SYMBIOTIC BACTERIA. Quantum Journal of Medical and Health Sciences, 1(1), 9–20. Retrieved from https://qjmhs.com/index.php/qjmhs/article/view/2