DNA BARCODING OF FEW INDOOR PLANTS AND MOLECULAR CHARACTERIZATION OF ITS SYMBIOTIC BACTERIA
Keywords:
DNA barcoding, indoor plants, symbiotic bacteria, isolation, identification, molecular characterizationAbstract
The health benefits of indoor plants are widely explored now a days and methods of psychological assessment are explored. In this regard we have collected few indoor plants and authenticated them to further investigate for its symbiotic relationship with bacteria. As traditional taxonomic studies possess intrinsic limitations with plant species identification, combinations of DNA barcodes have been considered a powerful tool to discover undetected genetic variation within species across large geographic areas, providing more precise estimates of biodiversity. Therefore, many genes have been targeted for DNA barcodes as standardizing regions is vital for identifying them efficiently and discriminate the plant species. In this study, we targeted different genes such as ITS2, matK, RbCl2 and PetB for sequencing. The plants were identified as Draceana senderiana,Pandanus sanderi and Hylotelephium ewersii. Once the species are authenticated they were further used to identify its bacterial relationship. The symbiotic bacteria present in roots were identified as Acinetobacter calcoaceticus and Pseudomonas stutzeri. This information will further help in exploring the properties of bacteria which are involved in enhancing the overall growth of plants.
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