IN VITRO ANTIOXIDANT AND ANTIMUTAGENIC ACTIVITY OF CURCUMIN AGAINST POTENT CHEMOTHERAPEUTIC AGENT DOXORUBICIN
Keywords:curcumin, micronucleus assay, doxorubicin, antioxidant property, DPPH method, peripheral leukocyte
The chemopreventive activity of several plants derived materials have been well studied in the recent years. Among them, Curcumin, the mostly investigated dietary phyto constituents have been shown the protective effect in cardiac, renal and hepatic toxicity induced by doxorubicin in human. The protective activity of any phytochemicals mainly depends on their antioxidant property. Thus, the present study was conducted to examine the antioxidant property of laboratory extracted and pure commercial curcumin and antimutagenic action of extracted and pure curcumin against the doxorubicin induced mutagenesis in human leukocyte. To analyze the mutagenicity, parameter like micronucleus assay was conducted in peripheral human leukocytes and to evaluate the antioxidant property, parameter like DPPH free radical scavenging activity was performed. The study results demonstrated that, both the extracted and pure form of curcumin (20µg/ml) induces micronucleus in human leukocytes in an insignificant manner when compared with negative control. Doxorubicin induces significant amount of micronucleus at two variable concentrations (0.50µg/ml and 0.25µg/ml). The micronucleus induction was reduced in human leukocytes pretreated by curcumin for 48 hours in both the concentrations of doxorubucin. Pure as well as extracted Curcumin has been shown strong free radical scavenging activity in a similar pattern as concentration dependant manner. The study reveals that curcumin has antimutagenic potential against chemotherapeutic agent like doxorubicin induced micronucleus in human leukocytes. In addition, it showed strong free radical scavenging activity by DPPH method by both extracted and pure form.
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