ANTIOXIDANT, PHENOLICS, PHYTOCHEMICAL, FLUORESCENCE PROFILE OF BAOBARANG (EMBELIA RIBES FRUITS): PROMISING UNANI IMMUNO PROTECTOR
Keywords:
unani, antioxidant, DPPH, total phenolic content, fluorescence, phytochemicalsAbstract
In unani medicine Embelia ribes fruits known as Baobarang are used to treat various diseases. Previous studies have shown them to possess Analgesic, Anti-microbial, Anti diabetic, Anti-cancer, anti-inflammatory activity etc. In view of the medicinal importance of Embelia ribes here, its fruit extracts in various solvents were evaluated for chemical constituents, total phenolics and in-vitro Antioxidant scavenging capacity by spectrophotometer. The Fluorescence profile of the herb was also generated by treatment with chemical reagents under UV (254 and 365 nm) and day light. The total phenolics in Aqueous, Alcoholic and hydroalcoholic extract were 46.66, 72.33 and 54.83 mg/g Gallic Acid Equivalent respectively and Antioxidant activity was determined using 2,2-diphenyl 1-picrylhydrazyl (DPPH)radical. Hydroalcoholic extract of Baobarang showed maximum scavenging activity with minimum IC50 value. Phytochemical analysis showed the presence of flavonoids, phenols, steroids, tannins, proteins, amino acids, carbohydrates, alkaloids, glycosides, resins and saponins. The presence of phenolics and antioxidant activity accounts for its use as potential herbal natural oxidative stress reducing remedy. The study further provides scientific support to the use of Baobarang as General tonic Anti-ageing and Immuno protective in unani system of medicine.
References
Annapurna, D., Srivastava, A., Rathore, T. S. (2013): Impact of Population Structure, Growth Habit and Seedling Ecology on Regeneration of Embelia ribes Burm. f.-Approaches toward a Quasi in Situ Conservation Strategy. – American Journal of Plant Sciences 4: 28-35.
Antoney, J., Britto, A.J.D., Abida, P., Raj, L.S. (2016): In-vitro cytotoxicity studies on methanolic leaf extract of Embelia ribes burm f-an important traditional medicinal plant of Kerala. – Advances in Cytology and Pathology 1(1): 6-8.
Bhandari, U., Kanojia, R., Pillai, K.K. (2002): Effect of ethanolic extract of Embelia ribes on dyslipidemia in diabetic rats. – Journal of Diabetes Research 3(3): 159-162.
Bhattacharjee, A.K., Das, A.K. (1969): Phytochemical screening of some Indian plants. – Quarterly Journal of Crude Drug Research 9(3): 1408-1412.
Brand-Williams, W., Cuvelier, M.E., Berset, C.L.W.T. (1995): Use of a free radical method to evaluate antioxidant activity. – LWT-Food Science and Technology 28(1): 25-30.
Chang, S.T., Wu, J.H., Wang, S.Y., Kang, P.L., Yang, N.S., Shyur, L.F. (2001): Antioxidant activity of extracts from Acacia confusa bark and heartwood. – Journal of Agricultural and Food Chemistry 49(7): 3420-3424.
Chase Jr, C.R., Pratt, R. (1949): Fluorescence of powdered vegetable drugs with particular reference to development of a system of identification. – Journal of the American Pharmaceutical Association 38(6): 324-331.
Fecka, I., Raj, D., Krauze-Baranowska, M. (2007): Quantitative determination of four water-soluble compounds in herbal drugs from Lamiaceae using different chromatographic techniques. – Chromatographia 66: 87-93.
Joshi, V.K., Joshi, A., Dhiman, K.S. (2017): The Ayurvedic Pharmacopoeia of India, development and perspectives. – Journal of Ethnopharmacology 197: 32-38.
Joy, B., Lakshmi, S. (2010): Antiproliferative properties of Embelia ribes. – The Open Process Chemistry Journal 3: 17-22.
Kähkönen, M.P., Hopia, A.I., Vuorela, H.J., Rauha, J.P., Pihlaja, K., Kujala, T.S., Heinonen, M. (1999): Antioxidant activity of plant extracts containing phenolic compounds. – Journal of Agricultural and Food Chemistry 47(10): 3954-3962.
Kalim, M.D., Bhattacharyya, D., Banerjee, A., Chattopadhyay, S. (2010): Oxidative DNA damage preventive activity and antioxidant potential of plants used in Unani system of medicine. – BMC Complementary and Alternative Medicine 10: 1-11.
Kokoski, C.J., Kokoski, R.J., Slama, F.J. (1958): Fluorescence of powdered vegetable drugs under ultraviolet radiation. – Journal of the American Pharmaceutical Association (Scientific ed.) 47(10): 715-717.
Krishnaswamy, M., Purushothaman, K.K. (1980): Antifertility properties of Embelia ribes:(embelin). – Indian Journal of Experimental Biologi 18(6): 638-639.
Lal, B., Mishra, N. (2013): Importance of Embelia ribes: An update. – International Journal of Pharmaceutical Sciences and Research 4(10): 3823-3838.
Mahendran, S., Thippeswamy, B.S., Veerapur, V.P., Badami, S. (2011): Anticonvulsant activity of embelin isolated from Embelia ribes. – Phytomedicine 18(2-3): 186-188.
Piluzza, G., Bullitta, S. (2011): Correlations between phenolic content and antioxidant properties in twenty-four plant species of traditional ethnoveterinary use in the Mediterranean area. – Pharmaceutical Biology 49(3): 240-247.
Rahman, K. (2007): Studies on free radicals, antioxidants, and co-factors. – Clinical Interventions in Aging 2(2): 219-236.
Saeed, N., Khan, M.R., Shabbir, M. (2012): Antioxidant activity, total phenolic and total flavonoid contents of whole plant extracts Torilis leptophylla L. – BMC Complementary and Alternative Medicine 12: 1-12.
Singh, A., Singh, D.K. (2001): Molluscicidal activity of Lawsonia inermis and its binary and tertiary combinations with other plant derived molluscicides. – Indian Journal of Experimental Biology 39(3): 263-268.
Singleton, V.L., Rossi, J.A. (1965): Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. – American Journal of Enology and Viticulture 16(3): 144-158.
Swamy, H.K., Krishna, V., Shankarmurthy, K., Rahiman, B.A., Mankani, K.L., Mahadevan, K.M., Harish, B.G., Naika, H.R. (2007): Wound healing activity of embelin isolated from the ethanol extract of leaves of Embelia ribes Burm. – Journal of Ethnopharmacology 109(3): 529-534.
Tambekar, D.H., Khante, B.S., Chandak, B.R., Titare, A.S., Boralkar, S.S., Aghadte, S.N. (2009): Screening of antibacterial potentials of some medicinal plants from Melghat forest in India. – African Journal of Traditional, Complementary and Alternative Medicines 6(3): 228-232.
Vinutha, B., Prashanth, D., Salma, K., Sreeja, S.L., Pratiti, D., Padmaja, R., Radhika, S., Amit, A., Venkateshwarlu, K., Deepak, M. (2007): Screening of selected Indian medicinal plants for acetylcholinesterase inhibitory activity. – Journal of Ethnopharmacology 109(2): 359-363.
Warrier, P.K. (1993): Indian medicinal plants: a compendium of 500 species. – Orient Blackswan 5: 592p.
Zutshi, U., Johri, R.K., Atal, C.K. (1989): Possible interaction of potassium embelate, a putative analgesic agent, with opiate receptors. – Indian Journal of Experimental Biology 27(7): 656-657.
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